| 1. | The amino acid analyzer could n ' t detect amino acid sequence with edman degradation , which meant apss had the blocked n - terminus
N一末端测序法不能分析apsi和” s5氨基酸序列，估计其n一末端封闭。 |
| 2. | The purification of this halocin was achieved by combination of tangential flow filtration ( tff ) , sephadex g50 and deae - sepharose chromatography . the n - terminal amino acid sequence was also determined by edman degradation
Halc8对大多数的极端嗜盐古菌(包括部分嗜盐碱古菌)有抑制作用，但对细菌则无明显的抑制作用。 |
| 3. | The complete amino acid sequences of them were obtained by automated edman degradation , and the biological functions of them have been investigated on several experimental models . they are named as jztx - and jztx - , respectively
Jztx -和jztx -的氨基酸序列全部测出，分别由34和36个氨基酸组成，含有6个半胱氨酸，形成3对二硫键。 |
| 4. | Among numerous strategies for c - terminal protein sequencing ( iso ) thiocyanate method established by schlack - kumpf in 1926 appears to be a promising approach because of the similarity to the edman degradation in mechanism
纵观蛋白质c端测序方法的发展，由于化学法中的(异)硫氰酸法反应机理与edman降解十分类似，方法，所以是最有前景的方法。 |
| 5. | However , due to the poor reactive carboxyl group of c - terminus , it is difficult to derivatize peptides topeptidyl t hiohydantoins for c - terminus , and the schlack - kumpf degradation based on thiohydantoin procedure has not yet been developed to such an efficient method as edman degradation
然而，由于c端羧基的化学性质不活泼，致使schlack - kumpf降解法的研究进展缓慢。但通过近十年的研究表明该方法是很有希望成为常规c端顺序测定的方法。 |
| 6. | 57 protein spots out of about 1000 detectable spots on the 2 - d gels were indentified by the following two methods : l ) n ~ terminal edman degradation microsequencing after the protein spots were electro - transferred to pvdf membrane . 2 ) maldi - tof - ms peptide fingerprint analysis of the protein spots and protein database searching . the 2d protein maps of the rice spikelet during the sterile and fertile stages were compared
二是通过原位酶解，抽提蛋白质片段进行maldi - tof质谱分析，利用肽质量指纹图数据在数据库中进行检索，在双向电泳凝胶上取了57个点进行分析，有34个蛋白质点在数据库得到归属鉴定。 |
